User:Anthony Salvagno/Notebook/Research/2009/07/01/E. coli Ready Lab

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Initial Email from Me to building Maint

We are looking into starting to work with E. coli. Koch mentioned that there might be some safety standards that are required for this. Do you know anything about this, or know who I could get in touch with that might?


Anthony, i'm forwarding your email to **** and srs- i'm sure they'll want to meet and go over whatever safety precautions we need to be taking; in the meantime, get us particulars on qty's, strains of E. coli and any other data that's appropriate.

My Response (via Koch)

It’s worth reminding him that what we’re doing is carried out in many other buildings on campus (Biology, Cancer Research Facility, etc.)—so we don’t have to reinvent the wheel. As for the other questions:

  • Stocks will be on the order of 1 milliliter, stored in -80C
  • When we produce protein or DNA, we’ll grow on the order of 1 liter of cells. The cells will be lysed (killed) and protein or DNA will be recovered with standard kits.
  • We expect to grow 1 liter of cells maybe once / month

Maint Reply (from a different person)

We definitely need to talk. You are planning on working with E.coli so I anticipate we will need to use a microbiology protocol for handling and disposal. How will you handle the waste stream from your 1 liter per month process? As Dan noted earlier, are you using any specially virulent varieties of E. coli? When can we meet? Next week perhaps? Do not bring in an E. coli culture until we define a handling and waste removal process.

My response

I will be out of town all next week. We can either do it the week after or tomorrow.

I wasn't planning on bringing anything in until I got this squared away. We weren't going to use anything too dangerous. I'm not entirely sure the specifics because I haven't worked on it yet, but we want a strain that can handle plasmid replication and possibly another strain that can transcribe the gene to make Kinesin.

I can also meet now.