User:Anthony Salvagno/Notebook/Research/2009/03/11/Official T'form Results
Results and Comments
We made 5 plates.SJK 17:12, 11 March 2009 (EDT)
|1||vector only||some background DNA, this was expected|
|2||insert only||nothing, good result|
|3||both vector and insert (1:1 V:I)||nothing, bad result|
|4||both vector and insert (2:1 V:I)||1 colony, funny result but still bad|
|5||nothing but E coli cells||nothing, good display of sterile technique|
We took that one colony and used it. We figured we might as well. I didn't want to waste all that effort I had put in.
- I plucked the colony
- Placed colony into LB media
- Place tube into shaker overnight
- Watch it grow.