User:Anthony Salvagno/Notebook/Research/2009/02/13

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/Plasmid Prep and Testing

We have some exciting experiments going on today. I recap the plan with using plasmids for cloning and tether construction, and discuss our preparation for those later steps. There is some endonucleasing going on in here...

/Gel Fabrication

I ran a gel. Here is my setup for it.

Things to do

  • Upload gel image
  • download pRS413 sequence and look for BstXI cutting sites.
  • Look up NotI cutting sites in yeast genome.