User:Andrew W Long/Notebook/Protein Modification/2014/12/01

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1-Pentanol

Reactions were run using procedure outlined on Nov 21 2014

Done Today:

  • Pentanol
    • KCl + protein in Phosphate
    • Protein+KCl in Tris
    • KCl+no protein in Phosphate
    • No protein+no KCl

For this next reaction and all reactions to follow, the concentration of OPD was modified. This was done with the hopes of developing a Michaelis-Menten curve. A solution of 1 mg OPD per 1 ml solvent was produced. 1 ml of that was taken, and placed in 9 ml of solvent. 2.025 ml of the dilute solution was then used for the reaction in the Ocean Optics.

  • Pentanol
    • KCl + protein in Phosphate
    • Protein+KCl in Tris
    • KCl+no protein in Phosphate
    • No protein+no KCl