User:Andrew W Long/Notebook/Protein Modification/2014/10/03

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Isobutyl Alcohol, Tris, KCl, Mb

Reaction performed using standard procedures outlined in the entry on September 22, 2014. Reaction was done with 15 mg of lyophilized KCl + protein in Tris buffer dissolved in Isobutyl Alcohol.


  • One identical reaction was carried out in a Falcon tube, and was allowed to rest undisturbed
    • Green layer began to form ~20-30 min into rxn
  • Another reaction was set up with an identical setup, but the solution was periodically pipetted up and down (~ every 10 min) to evenly mix (to prevent the formation of a distinct clear layer and a distinct green layer)

To Do List:

  • Isobutyl Alcohol, no KCl, no protein
  • Isobutyl Alcohol, KCl in Tris
  • Isobutyl Alcohol, KCl+protein in Tris