User:Andrew W Long/Notebook/CHEM-671 Research/2016/01/27

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Preparation of Stock Solutions, 1mL Reactions

Au solution

MW Au = 339.79 g/mol

Mass Au used = 50.44 mg = 0.05044 g

Moles of Au = 1.484*10^-4 mol Au

Volume of solution = 50 mL = 0.050 L

Concentration = 0.00297 mol/L Au


Lysozyme solution

MW = 14307 g/mol

Mass lysozyme used = 30.65 mg = 0.03065 g

Volume of solution = 100 mL = 0.100 L

Moles of lysozyme = 2.142*10^-6 mol lysozyme

Concentration = 4.285*10^-5 mol/L lysozyme


Preparation of reaction components for 1 mL samples

Target concentration of Au = 0.25 mM, Volume needed = 0.594 L

Target concentration of lysozyme 5.556 μM, Volume needed = 0.771 L

Volume of gold stock: 84.4 μL, Volume of protein stock: 259 μL, Volume of water: 656 μL