User:Andrew W Long/Notebook/CHEM-671 Research/2016/01/20

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Synthesis of AuNP-Lysozyme Fibers The original procedure can be found in Dr. Hartings's notebook. Stock solutions Au solution MW Au = 339.79 g/mol Mass Au used = 50.44 mg = 0.05044 g Moles of Au = 1.484*10^-4 mol Au Volume of solution = 50 mL = 0.050 L Concentration = 0.00297 mol/L Au Lysozyme solution MW = 14307 g/mol Mass lysozyme used = 30.65 mg = 0.03065 g Volume of solution = 100 mL = 0.100 L Moles of lysozyme = 2.142*10^-6 mol lysozyme Concentration = 4.285*10^-5 mol/L lysozyme Preparation of reaction components for 1 mL samples Target concentration of Au = 0.25 mM Volume needed = 0.594 L Target concentration of lysozyme 5.556 μM. Volume needed = 0.771 L Reaction volumes for 1 mL samples Volume of gold stock: 84.4 μL Volume of protein stock: 259 μL Volume of water: 656 μL