User:Andrew W Long/Notebook/CHEM-671 Research/2015/12/01

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ICP/UV-Vis/Conductivity Sample Prep

Today, 12 20mL AuNP fiber samples were prepared for tomorrow's examination using UV-Vis, conductivity probe, and ICP. All supernatant was siphoned, proteinase-K was added to each of the 12 containers in the following way:

Tubes 1-3: 1 uM proteinase-K

Tubes 4-6: 100 nM proteinase-K

Tubes 7-9: 10 nM proteinase-K

Tubes 10-12: 1 nM proteinase-K

Additionally, supernatant from tubes 1-3 was kept for later examination. The samples were allowed to incubate and digest over night, and were analyzed the following day.