User:Andrew K Farag/Notebook/CHEM-571/2014/03/18

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Objective

To begin collecting all data and based on our results refine the protocol for the rest of the semester.

Table of Volumes for Large Synthesis

80% BSA-Cysteine

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 40 mM Ammonium Acetate (uL) 10uM BSA* (uL) 10uM 1-Thioglycerol (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
80 10800 1200 12000 9600 2400 60000 12000 108

80% BSA-Cysteine, half concentration

% BSA 2.5 mM Zinc Nitrate (uL) 2.5 mM Manganese Acetate (uL) 20 mM Ammonium Acetate (uL) 5 uM BSA* (uL) 5 uM L-Cysteine (uL) 5 mM Ammonium Hydroxide (uL) 2.5 mM Sodium Sulfide* (uL) Total Volume (mL)
80 10800 1200 12000 9600 2400 60000 12000 108

80% BSA-GSH

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 40 mM Ammonium Acetate (uL) 10uM BSA* (uL) 10uM GSH (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
80 10800 1200 12000 9600 2400 60000 12000 108

80% BSA-1-Thioglycerol

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 40 mM Ammonium Acetate (uL) 10uM BSA* (uL) 10uM 1-Thioglycerol (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
80 10800 1200 12000 9600 2400 60000 12000 108

Preparation of Stock Solutions

' mass in grams (g) Final Volume (mL)
5mM Zinc Nitrate 0.0745 50
5mM Manganese Acetate 0.0123 10
40mM Ammonium Acetate 0.1541 50
10uM BSA 0.033 50
10mM Ammonium Hydroxide 168.92 (uL) 250
5mM Sodium Sulfide 0.06 50