User:Amit Sinha/Notebook/FirstProject/2010/08/02

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RNA Extraction and purification : RS2333 Dauers

  • Two dauers samples were obtained from liquid culture on 25 June 2010. Lysed in Trizol and stored at -80°C
  • Finished the extraction and purification using phenol-chloroform-isoamyl alcohol precipitation.
  • Quantification data from nanodrop:

2010.08.02.rs2333.dauer.pci.1.bmp 2010.08.02.rs2333.dauer.pci.2.bmp

  • Mix stage RNA from 05 April 2010 was used as reference RNA :