User:Allison K. Alix/Notebook/Thesis Research/2013/11/19
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1) Prepare the following dilutions of AuNP/DNA:
original concentration of AuNP: 290pM
3) Heat at ~78°C for 30 minutes
4) Cool to room temperature
The purpose of this analysis is to observe if fluorescence changes with changing concentrations of Au/DNA. Where as before one Au/DNA/MB solution was prepared and subsequent dilutions were made from that one solution, this procedure first prepares the dilutions and attaches the MB to each separate solution. The higher concentrations of Au/DNA have more binding sites for the beacon, so they are expected to bind fully. The lower concentrations should also bind fully with some closed MB in solution.