User:Allison K. Alix/Notebook/Thesis Research/2013/09/11

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FCS Measurements

  • To model the DNA-MB complex more specifically (and to understand if the lack of correlation curve from yesterday is due to sample or due to instrumentation) FCS measurements of an antibody-alexa 532 sample were taken. The following concentrations were prepared: 1000nM, 100nM, 10nM, 1 nM, 100pM, 10pM, 1pM. FCS measurements included those of the 1000pM, 100pM, and 10pM. No correlation curves were present. The 1000nM sample was used to verify a high count rate, but no fluorescence was visible on the camera.