User:Allison K. Alix/Notebook/Thesis Research/2013/09/04

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Absorbance Measurements of Au@SiO@/DNA (8/29/13)

Au@SiO2 DNA.png

ε of thiol-DNA= 607500 A=0.553 b=1 cm

A=εbc

c=A/εb

c = 9.10x10^-7M = 0.910uM

Addition of ThT

[ThT] must be twice what [DNA] is

[DNA] = 0.910μM, so [ThT] must be 1.82μM

Use 150μL of DNA-AuNP solution (10%=15μL)

1.820x10-6M x 165x10-6L solution = 3.003x10-10 moles ThT

(3.003x10-10 moles ThT)/ 15 x 10-6 L = 20.02μM

-Add 15μL of 20.2μM to 150μL thiol-DNA/AuNP solution

NEW Concentrations:

[DNA]= 0.827μM, [ThT] = 1.82μM

1) Prepare 100uL 20uM ThT (use already prepared 5/14/13)

2) React with DNA ten minutes

3) Observe absorbance and fluorescence

Absorbance Measurements of Au@SiO2/ThT-DNA

Fluorescence Measurements

Au@SiO2 DNA ThT.png

The intensity for this is much lower than expected, in comparison to the DNA/ThT samples that were not attached to any particles, BUT much higher than the DNA-ThT that was attached to the nanoparticles only. This might mean that the silica coating is indeed protecting fluorescence form being quenched. The next step is to run measurements on the FCS of different dilutions of this AuNP solution