User:Allison K. Alix/Notebook/Thesis Research/2013/08/29
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Revisions to DNA attachment on Au@SiO2
1) Combine 1mL 5nM silica coated Au particles with 5uL APS. Stir for 1 hour.
2) Add 2mg Sulfo-SMCC in 3mL KPS buffer (pH =7.4, 0.05M) add 1.5mL to NH2 functionalized AuNP's.
3) Stir for 1hr. and centrifuge for 30 seconds at a time at 9000rpm for three times. Remove supernatant and redisperse in 500uL KP buffer. Also centrifuge superntants and redisperse in ~30uL KP buffer. New volume of solution: 530uL
4) Add 270uL 1.5uM HS-DNA. Stir for 2 hours.