User:Allison K. Alix/Notebook/Thesis Research/2013/06/04

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Objectives

Procedures

1) Calibrate FCS with the following (both diluted 100x):

  • FluoSpheres carboxylate modified microspheres, 0.2μm, dark red fluorescent (excitation: 660nm, emission: 680nm)
  • FluoSpheres carboxylate modified microspheres, 0.1μm, orange fluorescent (excitation: 540nm, emission: 560nm)

(1/10)(x)=(1/100)(30μL) 3μL of 10x diluted in 27μL water for each

2) Run the following samples for 300s:

1. 1nM AuNP/140nM MB/74.94nM DNA

2. 500pM AuNP/70nM MB/37.47nM DNA

3. 100pM AuNP/14nM MB/7.49nM DNA

4. 140nM MB/80.27nM DNA

5. 70nM MB/40.13nM DNA

6. 14nM MB/8.03nM DNA

7. 140nM MB

8. 70nM MB

9. 14nM MB

Data

Sample <N> t (ms) <c> [nM]
dark red 24 0.3608 40
orange 813 121.465 1350
1 350 18.2086 581
2 302 5.6247 500
3 2.8 0.0033 4.6
4 1120 4.0469 1870
5 630 4.2236 1050
6 116 4.2980 193
7 480 2.3957 790
8 55.2 33.8102 91.7
9 100 0.0062 170