User:Allison K. Alix/Notebook/Thesis Research/2013/06/04

FCS Measurements of MB samples

Main project page Previous entry      Next entry

Objectives Take FCS Measurements of AuNP/DNA/MB, DNA/MB, and MB samples prepared on 6/3 (User:Allison K. Alix/Notebook/Thesis Research/2013/06/03) Procedures 1) Calibrate FCS with the following (both diluted 100x): FluoSpheres carboxylate modified microspheres, 0.2μm, dark red fluorescent (excitation: 660nm, emission: 680nm) FluoSpheres carboxylate modified microspheres, 0.1μm, orange fluorescent (excitation: 540nm, emission: 560nm) (1/10)(x)=(1/100)(30μL) 3μL of 10x diluted in 27μL water for each 2) Run the following samples for 300s: 1. 1nM AuNP/140nM MB/74.94nM DNA 2. 500pM AuNP/70nM MB/37.47nM DNA 3. 100pM AuNP/14nM MB/7.49nM DNA 4. 140nM MB/80.27nM DNA 5. 70nM MB/40.13nM DNA 6. 14nM MB/8.03nM DNA 7. 140nM MB 8. 70nM MB 9. 14nM MB Data Sample <N> t (ms) <c> [nM] dark red 24 0.3608 40 orange 813 121.465 1350 1 350 18.2086 581 2 302 5.6247 500 3 2.8 0.0033 4.6 4 1120 4.0469 1870 5 630 4.2236 1050 6 116 4.2980 193 7 480 2.3957 790 8 55.2 33.8102 91.7 9 100 0.0062 170