User:Allison K. Alix/Notebook/Thesis Research/2013/04/23

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Objectives Run 2% Gel with DNA-AuNP samples and supernatants Measure abs/fluor of thiol DNA/ThT Attach AuNP to thiol DNA/ThT Procedures Part 1: Run 2% agarose gel with the following samples: 1)5uL 100bp ladder + 1uL loading buffer 2)5uL DNA-AuNP + 1uL loading buffer 3)5uL sup 1 + 1uL loading buffer 4)5uL sup 2 + 1uL loading buffer 5)5uL sup 3 + 1uL loading buffer 6)5uL sup 4 + 1uL loading buffer Part 2: Take Absorbance and Fluorescence of thiol DNA-ThT sample Part 3: React thiol-DNA/ThT with AuNP Use procedure from 04/15/2013 original concentration of DNA in ThT: 2.91uM and 2.5uM after mixing with TEA: 1.45uM and 1.25uM after mixing with aunP: 727.5nM and 0.625uM ThT with 9.7nM AuNP Data