User:Alicia Rasines Mazo/Notebook/CHEM-571 Experimental Biological Chemistry/2014/09/17

Project name

Main project page Previous entry      Next entry

Tasks for September 17 To complete pH and conductivity readings To prepare samples for electrophoresis Preparation of Samples for Electrophoresis 1. Prepare electrophoresis samples and refrigerate until next week. <br.> For BSA, Lysozyme, [Au]:[BSA]=30:1 colloid, [Au]:[Lysozyme]=30:1 colloid and soy: Pipette 50μL of sample (for instance BSA stock or colloid solution) and dilute with 50 mM glycine stock solution to make up to 5 mL Take 10 μL of diluted solution and pour into 1.5 mL centrifuge Add 10 μL of running buffer Dr. Fox prepared Close centrifuge tube, vortex for 10 s and place into fridge Prepare Soy Protein Solution Dissolve 0.00209 g Soy Protein in 2 mL of H2O in volumetric flask. 50 μL of soy protein solution was diluted to 5 mL with 50 mM Glycine stock solution Take 10 μL of diluted solution (soy protein and glycine solution) and 10 μL running buffer and add to 1.5 mL centrifuge tube. Close lid, vortex for 5-10 seconds, and refrigerate. There are a total of 5 solution that are ready for electrophoresis next week.