User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/07/12

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AMPure Cleaned 454 Alien Gene Products

1) Combined 5.0ul of each culture #1-12 = 8 tubes 2) Cleaned with AMPure beads http://www.beckmangenomics.com/products/dna_purification_and_cleanup/agencourt_ampure_xp.html 3) Eluted with 40.0ul EB Buffer from Qiagen Extraction Kit.

Loaded 2.0ul on Gel

12July10.jpg

CULTURE PRIMER MIX TUBE
1 B1-F1 A
2 B2-F2 A
3 B3-F3 A
4 B4-F4 A
5 B5-F5 A
6 B6-F6 A
7 B7-F7 A
8 B8-F8 A
9 B9-F9 A
10 B10-F10 A
11 B11-F11 A
12 B12-F12 A
13 B1-F1 B
14 B2-F2 B
15 B3-F3 B
16 B4-F4 B
17 B5-F5 B
18 B6-F6 B
19 B7-F7 B
20 B8-F8 B
21 B9-F9 B
22 B10-F10 B
23 B11-F11 B
24 B12-F12 B
25 B1-F1 C
26 B2-F2 C
27 B3-F3 C
28 B4-F4 C
29 B5-F5 C
30 B6-F6 C
31 B7-F7 C
32 B8-F8 C
33 B9-F9 C
34 B10-F10 C
35 B11-F11 C
36 B12-F12 C
37 B1-F1 D
38 B2-F2 D
39 B3-F3 D
40 B4-F4 D
41 B5-F5 D
42 B6-F6 D
43 B7-F7 D
44 B8-F8 D
45 B9-F9 D
46 B10-F10 D
47 B11-F11 D
48 B12-F12 D
49 B1-F1 E
50 B2-F2 E
51 B3-F3 E
52 B4-F4 E
53 B5-F5 E
54 B6-F6 E
55 B7-F7 E
56 B8-F8 E
57 B9-F9 E
58 B10-F10 E
59 B11-F11 E
60 B12-F12 E
61 B1-F1 F
62 B2-F2 F
63 B3-F3 F
64 B4-F4 F
65 B5-F5 F
66 B6-F6 F
67 B7-F7 F
68 B8-F8 F
69 B9-F9 F
70 B10-F10 F
71 B11-F11 F
72 B12-F12 F
73 B1-F1 G
74 B2-F2 G
75 B3-F3 G
76 B4-F4 G
A B5-F5 G
B B6-F6 G
C B7-F7 G
D B8-F8 G
E B9-F9 G
F B10-F10 G
G B11-F11 G
H B12-F12 G
I B1-F1 H
CCA3 B2-F2 H
A. ricciae B3-F3 H

Yeast Culture Prep for Long Term Freezer Storage

From Revived Yeast Culture NCYC2722


1) Overnight culture on agar plate (or a few day until lawn is of sufficient size)

Streaked out 50.0ul of growing culture onto a glucose+yeast extract plate

S. pombe - failed to grow - redo S. cerevisiae - good lawn

2) Prepare freezing media = 20% gycerol in sterile YM media. Aliquot into small freezer tube (1000ul).

3) Collect lawn with sterile toothpick and swirl in freezing media

4) Slow freeze in -30C