User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/06/28

From OpenWetWare
Jump to: navigation, search
OpenWetWare photo.jpg Alien genes Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Library preparation for Alien genes

Adapter ligation

' conc. 1x
AGM (End Repaired) 10 ng/ul 5
Ligation buffer (Promega) 2 x 6.4
Atitin + adapter 50 uM 0.2
Btitn + adapter 50 uM 0.2
Ligase (Promega) 3U/ul 1
total 12.8
4C O/N