User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/06/25

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Adenita Alien Gene LongPCR

Re-Amp 19 and 21

' [CONC] 1X 6 ' ' AMP Lane Template MIX Contents ' Size Fosmid
Water 10.6 63.6 19 CCA3 111 AV10106 FWD AV10108.2 RVS 13400 AV_TEL_L_B
Takara LA Taq 5U 0.2 1.2 21 CCA3 55 AV10108 FWD AV10111 RVS 14846 AV_TEL_L_B
Takara LA Buffer 10X 2 12 CCA3
dNTP 2.5uM each 3.2 19.2
*Template 3.8ng 2 12
Primer Mix 5uM each 2
10.0ul 108 18.0ul/RXN
add 2.0 Primer Mix
* CCA3
PCR Cycle
94C 1 min
98C 10 sec
65C 15 min
30X

load 2.0 ul of sample

25June10.jpg

Library preparation for Alien genes

Fragmentation check by bioanalyzer

lane Sample conc. used
1 AGM end repaired 10 ng/ul 1 ul
2 AGM 1day ligaiton 33.8 ng/ul 1 ul
3 AGM 2days ligaiton 21.9 ng/ul 1 ul
4 AGM 3days ligaiton 31.0 ug/ul 1 ul

2100 expert High Sensitivity DNA Assay DE72901335 2010-06-25 10-07-58 Page 3.jpg 2100 expert High Sensitivity DNA Assay DE72901335 2010-06-25 10-07-58 Page 4.jpg 2100 expert High Sensitivity DNA Assay DE72901335 2010-06-25 10-07-58 Page 5.jpg Pages from 2100 expert High Sensitivity DNA Assay DE72901335 2010-06-25 10-07-58.jpg 2100 expert High Sensitivity DNA Assay DE72901335 2010-06-25 10-07-58 Page 6.jpg