User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/06/21

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Adenita Culture 454 Amplification Adapter extension

EX Takara RXN Mixture conc 1X 16 ' '
Milli Q water 6.15 98.4
Takara EX Buffer 10X 1 16
dNTP 2.5uM 0.8 12.8
Primer Mix 20uM 1
EX Taq 5U 0.05 0.8
Template Oleg PCR 1:10 1
PCR 1:5 128
8.0ul / RXN add 1.0ul Primer and 1.0ul PCR template
PCR cycle
98C 10 sec
60C 1 min
x15
AMP Lane Template Primer AMP Lane Template Primer
1 Culture 11 1:5 B11 1 Culture 11 1:10 B11
2 Culture 11 1:5 D11 2 Culture 11 1:10 D11
3 Culture 11 1:5 E11 3 Culture 11 1:10 E11
4 Culture 11 1:5 F11 4 Culture 11 1:10 F11
5 Culture H 1:5 B12 5 Culture H 1:10 B12
6 Culture H 1:5 C12 6 Culture H 1:10 C12
7 Culture H 1:5 E12 7 Culture H 1:10 E12
8 Culture H 1:5 F12 8 Culture H 1:10 F12

Load 2.0ul of sample


21June10.jpg