User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/06/09

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HCO LCO Cycle Sequencing Purification with QIAquick

For Culture 14 - failed injection

http://www.qiagen.com/products/dnacleanup/gelpcrsicleanupsystems/qiaquickpcrpurificationkit.aspx#Tabs=t2

    • see page 19.
    • 6ul product for cleaning. Resuspended in 10ul EB buffer.

Alien Gene PCR Purification with QIAquick

For previously Ethanol Cleaned samples 09April10

http://www.qiagen.com/products/dnacleanup/gelpcrsicleanupsystems/qiaquickpcrpurificationkit.aspx#Tabs=t2

    • see page 19.
    • ~8ul PCR product left for cleaning. Resuspended in 30ul EB buffer.
Sample ID Nucleic Acid Conc. Unit A260 A280 260/280 260/230 Sample Type Factor
1 14.5 ng/µl 0.29 0.149 1.95 1.68 DNA 50
2 12.3 ng/µl 0.245 0.122 2.01 2.21 DNA 50
3 7.7 ng/µl 0.153 0.064 2.4 1.81 DNA 50
4 16.3 ng/µl 0.327 0.17 1.92 2.27 DNA 50
5 11.4 ng/µl 0.228 0.109 2.09 1.79 DNA 50
6 15 ng/µl 0.3 0.152 1.97 1.83 DNA 50
7 7.5 ng/µl 0.149 0.067 2.24 2.03 DNA 50
8 34.8 ng/µl 0.697 0.385 1.81 2.01 DNA 50
9 30.8 ng/µl 0.617 0.341 1.81 1.99 DNA 50
10 5.8 ng/µl 0.116 0.062 1.86 1.74 DNA 50
11 14.2 ng/µl 0.285 0.147 1.93 1.83 DNA 50
12 10.9 ng/µl 0.219 0.102 2.15 1.86 DNA 50
13 8.9 ng/µl 0.179 0.094 1.91 2.32 DNA 50
14 12.9 ng/µl 0.258 0.136 1.89 2.05 DNA 50
15 44.4 ng/µl 0.889 0.489 1.82 1.8 DNA 50
16 11.1 ng/µl 0.222 0.128 1.73 1.63 DNA 50
17 11.5 ng/µl 0.23 0.119 1.92 2.21 DNA 50
18 11 ng/µl 0.221 0.107 2.07 2.17 DNA 50
19 6.1 ng/µl 0.122 0.061 1.99 1.71 DNA 50
20 6.4 ng/µl 0.128 0.062 2.08 2 DNA 50
21 5.4 ng/µl 0.109 0.067 1.63 1.56 DNA 50
22 10.2 ng/µl 0.204 0.121 1.68 1.96 DNA 50
23 15.3 ng/µl 0.306 0.175 1.75 1.56 DNA 50
24 15.3 ng/µl 0.306 0.164 1.87 1.57 DNA 50
25 10.3 ng/µl 0.206 0.1 2.05 1.91 DNA 50

Sample to add to mix

Sample MIX Contents ' Size Gene CCA3 Result Qiagen Cleaned ng/ul Sample Type Total Product Remaining bp/ng ul to mix ng added ul to mix x 5
1 23 AV10004 FWD AV10007 RVS 8691 AV212 A good 04Mar10 14.5 DNA x 29ul 0.001668393 1.091407102 15.82540299 5.457035512
2 1 AV10007 FWD AV10011 RVS 9262 AV212 A good 04Mar10 12.3 DNA x 29ul 0.001328007 1.371149132 16.86513433 6.855745662
3 2 AV10011 FWD AV10016 RVS 11924 AV212 A good 02Mar10 7.7 DNA x 29ul 0.000645756 2.81978678 21.71235821 14.0989339
4 24 AV10016 FWD AV10020 RVS 7020 AV212 A good 04Mar10 16.3 DNA x 29ul 0.002321937 0.7842139 12.78268657 3.921069499
5 25 AV10020 FWD AV10025 RVS 11202 AV212 A good 04Mar10 11.4 DNA x 29ul 0.001017675 1.789269442 20.39767164 8.946347211
6 4 AV10025 FWD AV10028 RVS 6843 AV212 A good 04Mar10 15 DNA x 29ul 0.002192021 0.830692537 12.46038806 4.153462687
7 34 AV10029 FWD AV10034 RVS 12093 AV240 B good 08Mar10 7.5 DNA x 29ul 0.000620194 2.93601194 22.02008955 14.6800597
8 40 AV10034 FWD AV10039 RVS 14180 AV240 B good 18Mar10 34.8 DNA x 29ul 0.002454161 0.741962601 25.82029851 3.709813004
9 50 AV10039 FWD AV10041 FWD 19555 AV240 B good 18Mar10 30.8 DNA x 29ul 0.001575045 1.156091297 35.60761194 5.780456484
10 30 EU643474 FWD EU643474 RVS 10062 AV240 B good 04Mar10 5.8 DNA x 29ul 0.000576426 3.158939784 18.32185075 15.79469892
11 31 AV10045 FWD AV10047 RVS 6762 AV240 B good 04Mar10 14.2 DNA x 29ul 0.00209997 0.867105318 12.31289552 4.335526592
12 32 AV10047 FWD AV10049 RVS 12719 AV240 B good 04Mar10 10.9 DNA x 29ul 0.000856986 2.124767904 23.15997015 10.62383952
13 33 AV10049 FWD AV10053 RVS 9353 AV240 B good 04Mar10 8.9 DNA x 29ul 0.000951566 1.913577059 17.03083582 9.567885293
14 78 AV10091 FWD AV10092.2 RVS 8127 AV TEL L A good 19Mar10 12.9 DNA x 29ul 0.001587302 1.147164179 14.79841791 5.735820896
15 85 AV10092.2 FWD AV10094 RVS 7036 AV TEL L A good 01Apr 44.4 DNA x 29ul 0.006310404 0.288554525 12.8118209 1.442772623
16 16 AV10094 FWD AV10098 RVS 8600 AV TEL L A good 02Mar10 11.1 DNA x 29ul 0.001290698 1.410783918 15.65970149 7.053919591
17 134 AV10098 FWD AV10104 FWD 8000 AV TEL L A&B good 01Apr 11.5 DNA x 29ul 0.0014375 1.266709929 14.56716418 6.333549643
18 51 AV10104 RVS AV10106 RVS 9618 AV_TEL_L_B good 16Mar 11 DNA x 29ul 0.001143689 1.59212483 17.51337313 7.960624152
19 111 AV10106 FWD AV10108.2 RVS 13400 AV_TEL_L_B good 29Mar 6.1 DNA x 29ul 0.000455224 4 24.4 20
20 112 AV10107.1 FWD AV10108 RVS 7900 AV TEL L B good 29Mar 6.4 DNA x 29ul 0.000810127 2.24766791 14.38507463 11.23833955
21 55 AV10108 FWD AV10111 RVS 14846 AV_TEL_L_B good 16Mar 5.4 DNA x 29ul 0.000363734 5.006113875 27.03301493 25.03056938
22 117 AV10111 FWD AV10113 RVS 8400 AV TEL L B good 29Mar 10.2 DNA x 29ul 0.001214286 1.499561018 15.29552239 7.497805092
23 128 AV10113 FWD TEL_L_B_3 RVS 11500 AV TEL L B good 01Apr 15.3 DNA x 29ul 0.001330435 1.368646961 20.94029851 6.843234806
24 131 AV10114 FWD TEL_L_B_4 RVS 8500 AV TEL L B good 01Apr 15.3 DNA x 29ul 0.0018 1.011608624 15.47761194 5.058043118
25 138 AV10083 FWD AV10084.2 RVS 5400 AV TEL L A good 05Apr10 10.3 DNA x 29ul 0.001907407 0.954644254 9.832835821 4.773221272