User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/26

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Nuclear Region Adenita Culture 454 Amplification

Re-Amp of 19May10 Re-Extracted Cultures 31, 32, 36, 37, 38 & 39

EX Takara RXN Mixture conc 1X 5 ' '
Milli Q water 4.15 20.75
Takara EX Buffer 10X 1 5
dNTP 2.5uM 0.8 4
Primer Mix 20uM 1
EX Taq 5U 0.05 0.25
Template Instagene 3 15
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C 10 sec
60C 1 min
x35
AMP Lane Template Primer AMP Lane Template Primer
1 31 Instagene B7 21 38 Instagene B2
2 31 Instagene C7 22 38 Instagene C2
3 31 Instagene D7 23 38 Instagene D2
4 31 Instagene E7 24 38 Instagene E2
5 31 Instagene F7 25 38 Instagene F2
6 32 Instagene B8 26 39 Instagene B3
7 32 Instagene C8 27 39 Instagene C3
8 32 Instagene D8 28 39 Instagene D3
9 32 Instagene E8 29 39 Instagene E3
10 32 Instagene F8 30 39 Instagene F3
11 36 Instagene B12 31 Neg Control B7
12 36 Instagene C12 32 Neg Control C7
13 36 Instagene D12 33 Neg Control D7
14 36 Instagene E12 34 Neg Control E7
15 36 Instagene F12 35 Neg Control F7
16 37 Instagene B1 36 Pos Control B7
17 37 Instagene C1 37 Pos Control C7
18 37 Instagene D1 38 Pos Control D7
19 37 Instagene E1 39 Pos Control E7
20 37 Instagene F1 40 Pos Control F7
*Positive Control = AV 20ng

Load 2.0ul Sample

26May10A.jpg

Nuclear Region Adenita Culture 454 Amplification

Re-Amp of 26May10 Re-Extracted Cultures 33, 34, 35, 40, 41, 42, 43

EX Takara RXN Mixture conc 1X 5 ' '
Milli Q water 4.15 20.75
Takara EX Buffer 10X 1 5
dNTP 2.5uM 0.8 4
Primer Mix 20uM 1
EX Taq 5U 0.05 0.25
Template Instagene 3 15
45
9.0ul / RXN add 1.0ul Primer
PCR cycle
98C 10 sec
60C 1 min
x35
AMP Lane Template Primer AMP Lane Template Primer
1 33 Instagene B9 21 41 Instagene B5
2 33 Instagene C9 22 41 Instagene C5
3 33 Instagene D9 23 41 Instagene D5
4 33 Instagene E9 24 41 Instagene E5
5 33 Instagene F9 25 41 Instagene F5
6 34 Instagene B10 26 42 Instagene B6
7 34 Instagene C10 27 42 Instagene C6
8 34 Instagene D10 28 42 Instagene D6
9 34 Instagene E10 29 42 Instagene E6
10 34 Instagene F10 30 42 Instagene F6
11 35 Instagene B11 31 Neg Control B9
12 35 Instagene C11 32 Neg Control C9
13 35 Instagene D11 33 Neg Control D9
14 35 Instagene E11 34 Neg Control E9
15 35 Instagene F11 35 Neg Control F9
16 40 Instagene B4 36 Pos Control B9
17 40 Instagene C4 37 Pos Control C9
18 40 Instagene D4 38 Pos Control D9
19 40 Instagene E4 39 Pos Control E9
20 40 Instagene F4 40 Pos Control F9
*Positive Control = AV 20ng

Load 2.0ul Sample

26May10B.jpg