User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/24

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Nuclear Region Adenita Culture 454 Amplification

Reaction mixture conc. 1x x61 ' lane Template Primer mix
Template ?? 3 183 1 Harvard A2 (#10) B10
ExTaq buffer 10x 1 61 2 C10
dNTP mix 2.5 mM each 0.8 48.8 3 D10
Primer mix 20 uM each 1 4 E10
ExTaq 5 U/ul 0.05 3.05 5 F10
Milli Q 4.15 253.15 6 Harvard A3 (#11) B11
10 ul 7 C11
8 D11
PCR cycle 9 E11
98C 10 sec 10 F11
60C 1 min 11 Ithaca 1 B1 (#17) B5
x35 12 C5
13 D5
14 E5
15 F5
16 Ithaca 1 B3 (#18) B6
17 C6
18 D6
19 E6
20 F6
21 Ithaca 1 B5 (#19) B7
22 C7
23 D7
24 E7
25 F7
26 Ithaca 1 C1 (#20) B8
27 C8
28 D8
29 E8
30 F8
31 Ithaca 1 D1 (#21) B9
32 C9
33 D9
34 E9
35 F9
36 Ithaca 2 A3 (#27) B3
37 C3
38 D3
39 E3
40 F3
41 Ithaca 2 A4 (#28) B4
42 C4
43 D4
44 E4
45 F4
46 Ithaca 2 A5 (#29) B5
47 C5
48 D5
49 E5
50 F5
51 MilliQ (negative control) B10
52 C10
53 D10
54 E10
55 F10
56 AV_HMW (20ug) B10
57 C10
58 D10
59 E10
60 F10

load 2.0 ul of sample

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