User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/05/06

Alien genes

Main project page Previous entry      Next entry

Nuclear Gene HS Takara PCR Optimization HS Takara RXN Mixture 3.0ul Instagene conc 1X 5.5 ' Milli Q water 3.1 17.05 Takara HS Buffer 10X 2 11 dNTP 2.5uM 0.8 4.4 Primer Mix 20uM 1 HS Taq 5U 0.1 0.55 Template Instagene 3 16.5 49.5 9.0ul / RXN add 1.0ul Primer HS Takara RXN Mixture 4.0ul Instagene conc 1X 5.5 Milli Q water 2.1 11.55 Takara HS Buffer 10X 2 11 dNTP 2.5uM 0.8 4.4 Primer Mix 20uM 4 HS Taq 5U 0.1 0.55 Template Instagene 4 22 49.5 9.0ul / RXN add 1.0ul Primer A PCR cycle 98C 10 sec 60C 1 min x35 AMP Lane Template Primer Amt of Template 1 3 Instagene A2 3 2 3 Instagene A3 3 3 3 Instagene A4 3 4 3 Instagene A5 3 5 3 Instagene A6 3 6 4 Instagene A2 3 7 4 Instagene A3 3 8 4 Instagene A4 3 9 4 Instagene A5 3 10 4 Instagene A6 3 11 3 Instagene A2 4 12 3 Instagene A3 4 13 3 Instagene A4 4 14 3 Instagene A5 4 15 3 Instagene A6 4 16 4 Instagene A2 4 17 4 Instagene A3 4 18 4 Instagene A4 4 19 4 Instagene A5 4 20 4 Instagene A6 4 21 AV 2ng A2 1 22 AV 2ng A3 1 23 AV 2ng A4 1 24 AV 2ng A5 1 25 AV 2ng A6 1 *AV 1ng Positive Control Load 2.0ul Sample AV 1ng control failed - bad DNA dilution Dye Dilution Test 1:10 Dye Stock : MilliQ Water From 06May10 PCR - samples 1-10