User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/04/29

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Nuclear Region 454 Amplification

EX Takara RXN Mixture conc 1X 6.5 '
Milli Q water 5.15 33.475
Takara EX Buffer 10X 1 6.5
dNTP 2.5uM 0.8 5.2
Primer Mix 20uM 1
EX Taq 5U 0.05 0.325
Template Instagene 2 13
58.5 9.0ul / RXN
A PCR cycle
98C 10 sec
60C 1 min
x35
Plate 3
Ithaca 2 A1 25
Ithaca 2 A2 26
Ithaca 2 A3 27
Ithaca 2 A4 28
Ithaca 2 A5 29
Pennsylvania (PA) B4 30
Tsukuba 1 A4 31
Tsukuba 1 A6 32
Tsukuba 1 B2 33
Tsukuba 1 B4 34
Tsukuba 1 C1 35
Tsukuba 1 C6 36
Plate 4
Tsukuba 3 D2 37
Tsukuba 3 D3 38
Tsukuba 3 D4 39
Woods Hole 1 A6 40
Woods Hole 1 C2 41
Woods Hole 1 C3 42
Yokohama B5 43
Baltimore A3 44
Baltimore A6 45
E. Windsor D4 46
Harvard A1 47
Ithaca 2 A6 48