Ethanol Post-PCR Clean Up
Procedure
1) For each 10 ul of PCR product, prepare the following mixture in strip-cap tube.
1 ul of 3M sodium acetate (NaOAc) pH 5.2
25 ul chilled absolute ethanol.
2) Transfer the PCR product (20ul) into the tube containing the mixture.
3) Vortex the tube and then store it at -20 for 30-50 min.
4) Spin for 30 min at maximum speed 14.000 rpm.
5) Rremove the supernatant carefully and don't disturb the pellet.
6) Wash the pellet with 70% ethanol. And spin the tube at maximum speed for 5 min.
7) Remove the supernatant and then dry the pellet – air dry.
8) Resuspend the pellet in 10 ul in sterile water.
LCH/HCO Products
| Sample ID
|
Nucleic Acid Conc.
|
Unit
|
A260
|
A280
|
260/280
|
260/230
|
Sample Type
|
| lco/hco 1 |
29.3 |
ng/µl |
0.586 |
0.394 |
1.49 |
0.88 |
DNA
|
| lco/hco 9 |
101.6 |
ng/µl |
2.031 |
1.215 |
1.67 |
2.16 |
DNA
|
| lco/hco 17 |
68 |
ng/µl |
1.359 |
0.876 |
1.55 |
1.29 |
DNA
|
| lco/hco 25 |
89.8 |
ng/µl |
1.797 |
1.17 |
1.54 |
1.32 |
DNA
|
| lco/hco 33 |
86.2 |
ng/µl |
1.724 |
1.144 |
1.51 |
1.28 |
DNA
|
| lco/hco 41 |
169.1 |
ng/µl |
3.382 |
2.087 |
1.62 |
1.34 |
DNA
|
| lco/hco 49 |
179 |
ng/µl |
3.579 |
2.207 |
1.62 |
1.58 |
DNA
|
| lco/hco 57 |
110.9 |
ng/µl |
2.217 |
1.43 |
1.55 |
1.33 |
DNA
|
| lco/hco 65 |
145 |
ng/µl |
2.9 |
1.798 |
1.61 |
1.51 |
DNA
|
| lco/hco 73 |
129.3 |
ng/µl |
2.586 |
1.619 |
1.6 |
1.45 |
DNA
|
Average Concentration = 110 ng/ul
| Source Plate
|
Source Plate Well
|
EX ID
|
| Baltimore |
A1 |
1
|
| Baltimore |
B1 |
2
|
| Baltimore |
C2 |
3
|
| Baltimore |
C3 |
4
|
| E. Windsor |
A1 |
5
|
| E. Windsor |
A3 |
6
|
| E. Windsor |
B1 |
7
|
| E. Windsor |
B2 |
8
|
| E. Windsor |
C2 |
9
|
| Harvard |
A2 |
10
|
| Harvard |
A3 |
11
|
| Ithaca 1 |
A1 |
12
|
| Ithaca 1 |
A2 |
13
|
| Ithaca 1 |
A3 |
14
|
| Ithaca 1 |
A5 |
15
|
| Ithaca 1 |
A6 |
16
|
| Ithaca 1 |
B1 |
17
|
| Ithaca 1 |
B3 |
18
|
| Ithaca 1 |
B5 |
19
|
| Ithaca 1 |
C1 |
20
|
| Ithaca 1 |
D1 |
21
|
| Ithaca 1 |
D3 |
22
|
| Ithaca 1 |
D4 |
23
|
| Ithaca 1 |
D6 |
24
|
| Ithaca 2 |
A1 |
25
|
| Ithaca 2 |
A2 |
26
|
| Ithaca 2 |
A3 |
27
|
| Ithaca 2 |
A4 |
28
|
| Ithaca 2 |
A5 |
29
|
| Pennsylvania (PA) |
B4 |
30
|
| Tsukuba 1 |
A4 |
31
|
| Tsukuba 1 |
A6 |
32
|
| Tsukuba 1 |
B2 |
33
|
| Tsukuba 1 |
B4 |
34
|
| Tsukuba 1 |
C1 |
35
|
| Tsukuba 1 |
C6 |
36
|
| Tsukuba 3 |
D2 |
37
|
| Tsukuba 3 |
D3 |
38
|
| Tsukuba 3 |
D4 |
39
|
| Woods Hole 1 |
A6 |
40
|
| Woods Hole 1 |
C2 |
41
|
| Woods Hole 1 |
C3 |
42
|
| Yokohama |
B5 |
43
|
| Baltimore |
A3 |
44
|
| Baltimore |
A6 |
45
|
| E. Windsor |
D4 |
46
|
| Harvard |
A1 |
47
|
| Ithaca 2 |
A6 |
48
|
| Pennsylvania (PA) |
D4 |
49
|
| Tsukuba 1 |
B5 |
50
|
| Yokohama |
A2 |
51
|
| Baltimore |
C1 |
52
|
| Ithaca 1 |
A4 |
53
|
| Pennsylvania (PA) |
B6 |
54
|
| Yokohama |
B2 |
55
|
| Yokohama |
A4 |
56
|
| Ithaca 1 |
C4 |
57
|
| Ithaca 1 |
D5 |
58
|
| Baltimore |
C5 |
59
|
| E. Windsor |
A2 |
60
|
| Pennsylvania (PA) |
B3 |
61
|
| Ithaca 1 |
C3 |
62
|
| Ithaca 1 |
C6 |
63
|
| Harvard |
A4 |
64
|
| Ithaca 1 |
B4 |
65
|
| Ithaca 1 |
C5 |
66
|
| Baltimore |
B2 |
67
|
| Baltimore |
B3 |
68
|
| Baltimore |
B6 |
69
|
| E. Windsor |
C3 |
70
|
| Pennsylvania (PA) |
D6 |
71
|
| Baltimore |
B5 |
72
|
| Ithaca 1 |
C2 |
73
|
| Pennsylvania (PA) |
D5 |
74
|
| Baltimore |
A5 |
75
|
| Pennsylvania (PA) |
B1 |
76
|
| Boston |
A1 |
A
|
| Boston |
A4 |
B
|
| Cape Cod (CC) |
A1 |
C
|
| Woods Hole 3 |
A5 |
D
|
| Woods Hole 1 |
B3 |
E
|
| Woods Hole 1 |
B5 |
F
|
| Woods Hole 1 |
C4 |
G
|
| Woods Hole 2 |
A6 |
H
|
| Woods Hole 3 |
C2 |
I
|
| Cape Cod (CC) |
A3 |
CCA3
|
Alien Gene LongPCR Ethanol Cleaned
| Ethanol Clean #
|
MIX
|
Contents
|
'
|
Size
|
Gene
|
CCA3 Result
|
Ethanol Cleaned ng/ul
|
Sample Type
|
| 1 |
23 |
AV10004 FWD |
AV10007 RVS |
8691 |
AV212 A |
good 04Mar10 |
495.3 |
DNA
|
| 2 |
1 |
AV10007 FWD |
AV10011 RVS |
9262 |
AV212 A |
good 04Mar10 |
437.4 |
DNA
|
| 3 |
2 |
AV10011 FWD |
AV10016 RVS |
11924 |
AV212 A |
good 02Mar10 |
314.9 |
DNA
|
| 4 |
24 |
AV10016 FWD |
AV10020 RVS |
7020 |
AV212 A |
good 04Mar10 |
408.3 |
DNA
|
| 5 |
25 |
AV10020 FWD |
AV10025 RVS |
11202 |
AV212 A |
good 04Mar10 |
403.1 |
DNA
|
| 6 |
4 |
AV10025 FWD |
AV10028 RVS |
6843 |
AV212 A |
good 04Mar10 |
330.8 |
DNA
|
| 7 |
34 |
AV10029 FWD |
AV10034 RVS |
12093 |
AV240 B |
good 08Mar10 |
380.5 |
DNA
|
| 8 |
40 |
AV10034 FWD |
AV10039 RVS |
14180 |
AV240 B |
good 18Mar10 |
1128.6 |
DNA
|
| 9 |
50 |
AV10039 FWD |
AV10041 FWD |
19555 |
AV240 B |
good 18Mar10 |
1888.1 |
DNA
|
| 10 |
30 |
EU643474 FWD |
EU643474 RVS |
10062 |
AV240 B |
good 04Mar10 |
206 |
DNA
|
| 11 |
31 |
AV10045 FWD |
AV10047 RVS |
6762 |
AV240 B |
good 04Mar10 |
393.3 |
DNA
|
| 12 |
32 |
AV10047 FWD |
AV10049 RVS |
12719 |
AV240 B |
good 04Mar10 |
423.1 |
DNA
|
| 13 |
33 |
AV10049 FWD |
AV10053 RVS |
9353 |
AV240 B |
good 04Mar10 |
364.3 |
DNA
|
| 14 |
78 |
AV10091 FWD |
AV10092.2 RVS |
8127 |
AV TEL L A |
good 19Mar10 |
515.5 |
DNA
|
| 15 |
85 |
AV10092.2 FWD |
AV10094 RVS |
7036 |
AV TEL L A |
good 01Apr |
1353.9 |
DNA
|
| 16 |
16 |
AV10094 FWD |
AV10098 RVS |
8600 |
AV TEL L A |
good 02Mar10 |
334.4 |
DNA
|
| 17 |
134 |
AV10098 FWD |
AV10104 FWD |
8000 |
AV TEL L A&B |
good 01Apr |
466.4 |
DNA
|
| 18 |
51 |
AV10104 RVS |
AV10106 RVS |
9618 |
AV_TEL_L_B |
good 16Mar |
430.3 |
DNA
|
| 19 |
111 |
AV10106 FWD |
AV10108.2 RVS |
13400 |
AV_TEL_L_B |
good 29Mar |
218.4 |
DNA
|
| 20 |
112 |
AV10107.1 FWD |
AV10108 RVS |
7900 |
AV TEL L B |
good 29Mar |
214.1 |
DNA
|
| 21 |
55 |
AV10108 FWD |
AV10111 RVS |
14846 |
AV_TEL_L_B |
good 16Mar |
210.7 |
DNA
|
| 22 |
117 |
AV10111 FWD |
AV10113 RVS |
8400 |
AV TEL L B |
good 29Mar |
355.8 |
DNA
|
| 23 |
128 |
AV10113 FWD |
TEL_L_B_3 RVS |
11500 |
AV TEL L B |
good 01Apr |
422.7 |
DNA
|
| 24 |
131 |
AV10114 FWD |
TEL_L_B_4 RVS |
8500 |
AV TEL L B |
good 01Apr |
374.2 |
DNA
|
|
Alien genes
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