User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/04/02

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Alien Genes New Primer Screen

Reaction mixture conc. 1x x32.5 ' Lane Gene Lane Gene
Temp (Pennsylvania (PA) B3, 61) ?? 2 65 1 AV10158 17 AV10084
TopTaq Buffer 10x 1 32.5 2 AV10153 18 AV10082
dNTP mix 10 mM each 0.2 6.5 3 AV10151 19 AV10075
Primer mix 5 uM each 1 4 AV10150 20 AV10071
TopTaq 5 U/ul 0.05 1.625 5 AV10140 21 AV10045
Milli Q 5.75 186.875 6 AV10134 22 AV10043
10 ul 7 AV10130 23 AV10042
8 AV10123 24 AV10041
PCR cycle 9 AV10121 25 AV10034
94C 3 min 10 AV10104 26 AV10028
11 AV10109 27 AV10027
98C 10 sec 12 AV10099 28 AV10025
60C 3 min 13 AV10098 29 AV10016
x35 14 AV10094 30 AV10011
15 AV10092 31 AV10007
72C 5 min 16 AV10089 32 AV10002

Loaded 2.5ul of each sample

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Apis mellifera methylome

Mature-worker_sonicated_by_Covaris

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Callow-worker_sonicated_by_Covaris

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