User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/30

From OpenWetWare
Jump to: navigation, search
OpenWetWare photo.jpg Alien genes <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Alien Genes New Primer Screen

Reaction mixture conc. 1x x97 ' No. Primer mix
Temp (49 - 60) ?? 2 194 1 AV10134
TopTaq Buffer 10x 1 97 2 AV10121
dNTP mix 10 mM each 0.2 19.4 3 AV10084
Primer mix 5 uM each 1 4 AV10045
TopTaq 5 U/ul 0.05 4.85 5 AV10044
Milli Q 5.75 557.75 6 AV10034
10 ul 7 AV10027
8 AV10025
PCR cycle
94C 3 min No.
27 Ithaca 2 A3
98C 10 sec 28 Ithaca 2 A4
60C 3 min 29 Ithaca 2 A5
x35 37 Tsukuba 3 D2
40 Woods Hole 1 A6
72C 5 min 43 Yokohama B5
47 Harvard A1
48 Ithaca 2 A6
49 Pennsylvania (PA) D4
50 Tsukuba 1 B5
51 Yokohama A2
55 Yokohama B2


loaded 2.0ul of each sample

0056.jpg


Apis mellifera methylome

Sample (Honey bee brain) ' Program
Callow worker 6.1 ug/100ul Target Base Pair (Peak) 300bp
Mature worker 3.8 ug/100ul
Duty Cycle 10%
Intensity 4
Cycle per Burst 200
Time (second) 120
Chiller Temperature 19.5C

Loaded 5.0ul/100.0ul of each sample

0054.jpg