User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/10

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Alien Genes New Primer Screen

Reaction mixture conc. 1x 6
Template CCA3 1ng/ul 1 6
TopTaq buffer 10x 1
dNTP mix 10 mM each 0.2 1.2
Primer mix 5 uM each 0.5
TopTaq (QIAGEN) 5U/ul 0.05 0.3
Milli Q water 7.25 43.5
10 ul
PCR cycle
94C 3 min
98C 10 sec
60C 3 min
x35
72C 5 min

Load 5.0ul sample File:10march10.tif

AMP Lane Contents Fosmid
1 AV10030 AV240_B
2 AV10036 AV240_B
3 AV10037 AV240_B
4 AV10039 AV240_B
5 POS Control AV10008