User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/09

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Alien Gene New Primer Screen

Reaction mixture conc. 1x 12
Template CCA3 1ng/ul 1 12
TopTaq buffer 10x 1 12
dNTP mix 10 mM each 0.2 2.4
Primer mix 5 uM each 0.5
TopTaq (QIAGEN) 5U/ul 0.05 0.6
Milli Q water 7.25 87
10 ul
PCR cycle
94C 3 min
98C 10 sec
60C 3 min
x35
72C 5 min


Load 5.0ul sample File:09march10.tif

AMP Lane Contents Fosmid
1 AV10081 AV_TEL_L_A
2 AV10086 AV_TEL_L_A
3 AV10091 AV_TEL_L_A
4 AV10092.2 AV_TEL_L_A
5 AV10093.1 AV_TEL_L_A
6 AV10093.2 AV_TEL_L_A
7 AV100100 AV_TEL_L_A
8 AV10106 AV_TEL_L_B
9 AV10108 AV_TEL_L_B
10 AV10111 AV_TEL_L_B
11 POS Control AV10008