User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/03

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Alien Genes New Primer Screen

Screened on CCA3

Reaction mixture conc. 1x 16
Template 1ng/ul 1 16
TopTaq buffer 10x 1 16
dNTP mix 10 mM each 0.2 3.2
Primer mix 5 uM each 0.5
TopTaq (QIAGEN) 5U/ul 0.05 0.8
Milli Q 7.25 116
10 ul
PCR cycle
94C 3 min
98C 10 sec
60C 3 min
x35
72C 5 min

Loaded 1.0ul of sample File:04march10.tif

AMP Lane Primer
1 AV10004
2 AV10008
3 AV10010
4 AV10020
5 AV10022
6 AV10029
7 AV10032
8 AV10040.1
9 AV10040.2
10 AV10047
11 AV10049
12 AV10053
13 AV10087
14 AV10095
15 AV10114

Alien Genes New Primer Screen

Lane Gene Lane Gene
1 AV10158 17 AV10084
2 AV10153 18 AV10082
3 AV10151 19 AV10075
4 AV10150 20 AV10071
5 AV10140 21 AV10045
6 AV10134 22 AV10043
7 AV10130 23 AV10042
8 AV10123 24 AV10041
9 AV10121 25 AV10034
10 AV10104 26 AV10028
11 AV10109 27 AV10027
12 AV10099 28 AV10025
13 AV10098 29 AV10016
14 AV10094 30 AV10011
15 AV10092 31 AV10007
16 AV10089 32 AV10002
Reaction mixture conc. 1x x33
Temp (Ithaca1 B5,C1,D1) ?? 2 66
TopTaq buffer 10x 1 33
dNTP mix 10 mM each 0.2 6.6
Primer mix 5 uM each 0.5
TopTaq (QIAGEN) 5U/ul 0.05 1.65
Milli Q 6.25 206.25
10 ul
PCR cycle
94C 3 min
98C 10 sec
60C 3 min
x35
72C 5 min

Loaded 2.0ul of sample

3Mar'10 Ithaca1(B5,C1,D1).jpg

Reaction mixture conc. 1x x33
Temp (Ithaca1 D3,D4,D6) ?? 2 66
TopTaq buffer 10x 1 33
dNTP mix 10 mM each 0.2 6.6
Primer mix 5 uM each 0.5
TopTaq (QIAGEN) 5U/ul 0.05 1.65
Milli Q 6.25 206.25
10 ul
PCR cycle
94C 3 min
98C 10 sec
60C 3 min
x35
72C 5 min

Loaded 2.0ul of sample

3Mar'10 Ithaca1(D3,D4,D6).jpg

Reaction mixture conc. 1x x33
Temp (Ithaca2 A1,A2,A3) ?? 2 66
TopTaq buffer 10x 1 33
dNTP mix 10 mM each 0.2 6.6
Primer mix 5 uM each 0.5
TopTaq (QIAGEN) 5U/ul 0.05 1.65
Milli Q 6.25 206.25
10 ul
PCR cycle
94C 3 min
98C 10 sec
60C 3 min
x35
72C 5 min

Loaded 2.0ul of sample

3Mar'10 Ithaca2(A1,A2,A3).jpg