User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/02/22

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Alien Genes LongPCR

Amplification of AV_TEL_L_A & AV_TEL_L_B

' [CONC] 1X 5X '
Water 5.3 26.5
Takara LA Taq 5U 0.1 0.5
Takara LA Buffer 10X 1 5
dNTP 2.5uM each 1.6 8
Template 1ng 1 5
Primer Mix 5uM each 1
10.0ul 45 / 5 = 9.0ul/ RXN
add 1.0 Primer Mix
PCR Cycle
94C 1 min
98C 10 sec
68C 15 min
30X


Loaded 2.0ul of sample with 2.0ul dye File:22Feb10 Long PCR.tif

Lane Template Contents ' Fosmid Mix
1 AV AV10082 FWD AV10084 RVS AV_TEL_L_A 8
2 AV AV10092 FWD AV10094 RVS AV_TEL_L_A 11
3 AV AV10094 FWD AV10099 RVS AV_TEL_L_A 12
4 AV AV10104 FWD AV10109 RVS AV_TEL_L_B 13
5 CCA3 AV10082 FWD AV10084 RVS AV_TEL_L_A 8
6 CCA3 AV10092 FWD AV10094 RVS AV_TEL_L_A 11
7 CCA3 AV10094 FWD AV10099 RVS AV_TEL_L_A 12
8 CCA3 AV10104 FWD AV10109 RVS AV_TEL_L_B 13