User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/02/19

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Contents

HCO LCO

Amplification of HCO LCO

' [CONC] 1X 20X '
Water 6.05 121
Takara R Buffer 10X 1 20
MgCl2 25uM 0.6 12
dNTP 2.5uM each 0.8 16
Primer Mix LCO/HCO 5uM each 0.5 10
Takara R Taq 5U 0.05 1
Template InstaGene 1
180ul / 20 9ul / RXN
PCR Cylce '
94C 30 sec
45C 30 sec
72C 1 min
30X

Loaded 2.0ul of sample with 100BP Ladder

File:19Feb10 LCO HCO PCR.tif

AMP Lane Template '
1 Baltimore A1
2 Baltimore B1
3 Baltimore C2
4 Baltimore C3
5 E. Windsor A1
6 E. Windsor A3
7 E. Windsor B1
8 E. Windsor B2
9 E. Windsor C2
10 Harvard A2
11 Harvard A3
12 Ithaca 1 A1
13 Ithaca 1 A2
14 Ithaca 1 A3
15 Ithaca 1 A5
labeled Cape Cod A3
labeled AV