User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/02/17

From OpenWetWare
Jump to: navigation, search
OpenWetWare photo.jpg Alien genes <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Alien Genes LongPCR

Amplification of AV212_A

' [CONC] 1X 5X
Water 5.3 26.5
Takara LA Taq 5U 0.1 0.5
Takara LA Buffer 10X 1 5
dNTP 2.5uM each 1.6 8
Template * 1 5
Primer Mix 5uM each 1
10.0ul 45 / 5 = 9.0ul/ RXN
add 1.0 Primer Mix
* CCA3 0.2ng
*AV 0.5ng
PCR Cycle
94C 1 min
98C 10 sec
68C 15 min
30X
72C 10 min
Lane Template Contents ' Fosmid Mix
1 CCA3 AV10016 FWD AV10027 RVS AV212_A 5
2 CCA3 AV10016 FWD AV10028 RVS AV212_A 6
3 AV AV10016 FWD AV10027 RVS AV212_A 5
4 AV AV10016 FWD AV10028 RVS AV212_A 6

Load 2.0ul sample File:17Feb10 Long PCR.tif