User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/02/15

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Alien Genes LongPCR

Amplification of AV212_A

Test Primers on Adineta vaga DNA

' [CONC] 1X 5X
Water 26.5 26.5
Takara LA Taq 5U 0.1 0.5
Takara LA Buffer 10X 1 5
dNTP 2.5uM each 1.6 8
Template 20ng/ul 1 5
Primer Mix 5uM each 1
10.0ul 45 / 5 = 9.0ul/ RXN
add 1.0 Primer Mix
PCR Cycle
94C 1 min
98C 10 sec
68C 15 min
30X
72C 10 min
Lane Contents ' ' '
1 100BP ladder Fosmid Mix
2 AV10007 FWD AV10011 RVS AV212_A 1
3 AV10011 FWD AV10016 RVS AV212_A 2
4 AV10016 FWD AV10025 RVS AV212_A 3
5 AV10025 FWD AV10028 RVS AV212_A 4

Loaded 3ul PCR product

  • Well 2,3,4 showed some evaporation

File:15Feb10 Long PCR.tif