User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/18

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Cycle Sequencing Adineta Primer 1-36 Alien Genes

Cycle Sequencing ' ' '
Reagents Concentration 1X 100X
BDT 0.5 50
DMSO 0.1 10
5X Seq Buffer 5X 0.4 40
H20 3.6 360
*Primer 15uM 0.4
*PEG DNA variable 1
TOTAL 6 460
Cocktail per well = 4.6ul
*add primer and PEG separately
PCR 96C 10 sec
50C 5 sec
60C 4 min
60 cycles
Primers Sequence FWD and RVS
NAME Primer # NAME Primer # NAME Primer #
AV10158 1 AV10098 13 AV10034 25
AV10153 2 AV10092 14 AV10028 26
AV10151 3 AV10089 15 AV10027 27
AV10150 4 AV10084 16 AV10025 28
AV10140 5 AV10082 17 AV10016 29
AV10134 6 AV10075 18 AV10011 30
AV10130 7 AV10071 19 AV10007 31
AV10123 8 AV10045 20 AV10002 32
AV10121 9 AV10044 21 5475F+8047R 33
AV10104 10 AV10043 22 4220F+7822R 34
AV10109 11 AV10042 23 AV10094F2+R2 35
AV10099 12 AV10041 24 HISR1+F1 36
WELL 1 2 3 4 5 6
A AV10153_Boston_A1 AV10130_Boston_A4 AV10123_Boston_A4 AV10104_WH1_B3 AV10089_WH1_B3 AV10075_CC_A3
B AV10153_WH1_C4 AV10130_CC_A1 AV10123_WH1_B3 AV10104_WH1_B5 AV10089_WH1_B6 AV10045_CC_A3
C AV10150_CC_A3 AV10130_CC_A3 AV10123_WH1_B5 AV10104_WH1_B6 AV10089_WH3_C2 AV10045_WH3_A5
D AV10151_CC_A3 AV10130_WH1_B3 AV10123_WH1_B6 AV10104_WH1_C4 AV10084_CC_A3 AV10044_CC_A3
E AV10140_CC_A3 AV10130_WH1_B5 AV10123_WH3_C2 AV10109_CC_A3 AV10084_WH1_B6 AV10043_CC_A3
F AV10134_CC_A1 AV10130_WH1_B6 AV10121_CC_A3 AV10098_Boston_A1 AV10084_WH3_C2 AV10042_CC_A3
G AV10134_WH3_C2 AV10130_WH3_C2 AV10104_CC_A1 AV10098_Boston_A4 AV10082_WH1_B5 AV10042_WH1_B6
H AV10130_Boston_A1 AV10123_Boston_A1 AV10104_CC_A3 AV10098_CC_A3 AV10082_WH1_B6 AV10042_WH3_A5
WELL 7 8 9 10 11 12
A AV10041_Boston_A4 AV10027_CC_A3 AV10025_WH1_B6 5475_WH1_C4 AV100942_WH1_C4 HIS1_WH1_B5
B AV10041_CC_A3 AV10027_WH2_A6 AV10016_CC_A3 5475_WH3_A5 AV100942_WH3_A5 HIS1_WH1_B6
C AV10034_CC_A1 AV10027_WH3_A5 AV10011_CC_A3 5475_WH3_C2 AV100942_WH3_C2 HIS1_WH1_C4
D AV10034_CC_A3 AV10027_WH3_C2 AV10007_CC_A3 AV100942_CC_A1 HIS1_Boston_A1 HIS1_WH2_A6
E AV10028_CC_A3 AV10025_CC_A1 5475_CC_A3 AV100942_CC_A3 HIS1_Boston_A4 HIS1_WH3_A5
F AV10028_WH1_B3 AV10025_CC_A3 5475_WH1_B3 AV100942_WH1_B3 HIS1_CC_A1 HIS1_WH3_C2
G AV10027_Boston_A4 AV10025_WH1_B3 5475_WH1_B5 AV100942_WH1_B5 HIS1_CC_A3 Sample
H AV10027_CC_A1 AV10025_WH1_B5 5475_WH1_B6 AV100942_WH1_B6 HIS1_WH1_B3 Sample