User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/24

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To do Extractions of A. vaga test extraction volumes Extraction 10, 20, 40 A. vaga in 200 ul GeneMatrix standard heating scheme, with periodic agitation during extraction PCR Reagents 1x conc. 4 h2o 5.75 - 23 10x 1 10x 4 dye 1 10x 4 dNTP 0.2 10 mM 0.8 Primers 0 5 uM 4 Taq 0.05 5 U/um 0.2 DNA 2 5 ng/ul TOTAL 10 Cocktail per vial 9 3m 95C (10s 95C, 1m 60C)x35 + (40 ng) 10 20 40