User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/23

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Try re-dissolving failed primers AV10126 (B03 B04) and AV10094 (C05 C06) that failed previously

Reagents 1x conc. 6
h2o 6.75 - 40.5
10x 1 10x 6
dye 1 10x 6
dNTP 0.2 10 mM 1.2
Forward 0 10 uM
Taq 0.05 5 U/um 0.3
DNA 1 5 ng/ul
TOTAL 10 Cocktail per vial 9
  • 3m 95C (10s 95C, 60C 1m)x35
  • first three wells -- a test extraction (1ul of single rotifer in 40 ul GeneMatrix)
  • second three wells, re-duluted primers, with a positive control in the first well.
Gid 2009-11-23 20hr 12mina.jpg
  • need to re-design primers