User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/09

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Alien genes <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

To do

  • Organize rotifers
  • Order:
    • competent cells
    • frag enzyme from NEB

PCR of Juno2 from ligated DNA

Reagents 1x conc. 4x
h2o 15.05 - 60.2
10x 2.5 10x 10
dNTP 1.25 10 mM 5
Linker 1 10 uM
Juno 1 10 uM
Taq 0.2 5 U/um 0.8
DNA 4 4.4ng (~.45 fmol)/ul 16
TOTAL 25 Cocktail per vial 23
3m 93C (15s 93C; 15m 60C)x20
  • primer combinations:
Juno2_F Juno2_F Juno2_R Juno2_R
Linker_F Linker_R Linker_F Linker_R

Gid 2009-11-09 17hr 41min.jpg