User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/09

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To do

  • Organize rotifers
  • Order:
    • competent cells
    • frag enzyme from NEB

PCR of Juno2 from ligated DNA

Reagents 1x conc. 4x
h2o 15.05 - 60.2
10x 2.5 10x 10
dNTP 1.25 10 mM 5
Linker 1 10 uM
Juno 1 10 uM
Taq 0.2 5 U/um 0.8
DNA 4 4.4ng (~.45 fmol)/ul 16
TOTAL 25 Cocktail per vial 23
3m 93C (15s 93C; 15m 60C)x20
  • primer combinations:
Juno2_F Juno2_F Juno2_R Juno2_R
Linker_F Linker_R Linker_F Linker_R

Gid 2009-11-09 17hr 41min.jpg