User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/08

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Contents

To do

  • Organize rotifers
  • Check to see whether PCR product from yesterday is a juno2 transposon

PCR

Reagents 1x conc. 3
h2o 5.95 - 17.85
10x 1 10x 3
dye 1 10x 3
dNTP 0.2 10 mM 0.6
Juno2_F 0.4 10 uM 1.2
Juno2_R 0.4 10 uM 1.2
Taq 0.05 5 U/um 0.15
DNA 1 5 ng/ul
TOTAL 10 Cocktail per vial 9
3m 95C (15s 94C; 2m 60C)x30
  • templates
    • 1:100 dilution of yesterday's PCR product
    • P. rapida genomic
    • P. roseola genomic (+ control)

Gid 2009-11-08 15hr 07min.jpg