User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/08

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To do Organize rotifers Check to see whether PCR product from yesterday is a juno2 transposon PCR Reagents 1x conc. 3 h2o 5.95 - 17.85 10x 1 10x 3 dye 1 10x 3 dNTP 0.2 10 mM 0.6 Juno2_F 0.4 10 uM 1.2 Juno2_R 0.4 10 uM 1.2 Taq 0.05 5 U/um 0.15 DNA 1 5 ng/ul TOTAL 10 Cocktail per vial 9 3m 95C (15s 94C; 2m 60C)x30

• templates
  • 1:100 dilution of yesterday's PCR product
  • P. rapida genomic
  • P. roseola genomic (+ control)