User:Alexander S Mikheyev/Notebook/oil flies/2009/12/13

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Oil flies Report.pngMain project page
Resultset previous.pngPrevious entry      

PCR of oil fly bacteria

reagents 1x 15x
water 6.1 91.5
5x 7.5 112.5
1046R 0.3 4.5
917F 0.3
2.5 U/ul Platinum HiFi Pol 0.3 4.5
template 0.5
total 15


DNA 917F DNA 917F
pit91 guts TCGAG pit91 skin ACTCG
pit91 guts TCGAG pit91 skin ACTCG
pit91 guts TCGAG pit91 skin ACTCG
small pit guts CACAC small pit skin ATACG
small pit guts CACAC small pit skin ATACG
small pit guts CACAC small pit skin ATACG
negative all 917F
  • 94C 3 min (94C 10s; 57C 45s; 72C 1m)x 20 cycles