User:Alexander S Mikheyev/Notebook/mutation accumulation in rotifers/2009/12/04

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Test InstaGene Extraction Quality

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Plate Well Genus 12/2/2009 Count ' Reagents Concentration 1X 15X
Adenita 23Nov09 B3 Adineta 30+ H20 4.75 71.25
AV 10-06-09 well 6 Adineta 100+ 10X Buffer 10X 1 15
Boston A1 Adineta 10+ dye 10X 1 15
Boston A4 Adineta 50+ dNTP 10mM 0.2 3
CC A1 Adineta subs 100+ primer mix 5uM 1 15
CC A3 Adineta subs 100+ Taq 5U/uM 0.05 0.75
WH1 B3 Adineta 20+ DNA variable 2
WH1 B5 Adineta 20+ TOTAL 10 120
WH1 B6 Adineta 50+ Cocktail per well = 8.0ul
WH1 C4 Adineta 30+
WH2 A6 Adineta 10+ Primer HIS F1+R1
WH3 A5 Adineta 20+
WH3 C2 Adineta 7 PCR 95C 3 min
95C 10 sec
EXTRACTION 60C 2 min
5-10 individuals 35 cycles
InstaGene Matrix 200ul per sample
56C 30 min
99.9C 8min