User:Alexander S Mikheyev/Notebook/mutation accumulation in rotifers

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<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext">11/02/2009,11/03/2009,12/04/2009</div><div style="display:none;" id="page">User:Alexander S Mikheyev/Notebook/mutation accumulation in rotifers</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

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Project Description/Abstract

  • This project aims to address several questions using the same experimental framework (mutation accumulation lines).
    • What is the mutation rate of rotifer genomes?
    • Does dessication play a role in increasing genetic diversity of bdelloid rotifer populations? (Maybe we'll get to that)

Here is the basic plan

  • It takes one about a day for P. roseola eggs to hatch, and about 5 for the rotifer to become sexually mature.
  • Take offspring of a single young female P. roseola and culture them in 24 well dishes.
  • After 5 days take one young rotifer female and from each well and move her to a plate.

Notes

  • Doing pilot tests of P. roseola lab culture life history


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