User:Alexander S Mikheyev/Notebook/P. rapida partial genome/2009/12/07

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Owwnotebook icon.png P.rapida genome Report.pngMain project page
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QC ligation of the clones

water 3.3
buffer 0.5
dntp 0.1
taq 0.1
dna 1
  • 45min @ 72C, proceed to ligation
reagent volume conc molarity
2x 1.666666667
vector 0.333333333 50 ng/ul 26 fmol/ul
pcr 1 6 ng/ul 61 fmol/ul
t4 0.333333333 3U/ul
total 3.333333333
  • same reacti
  • ligation for 4 hours at 16C