User:Alexander S Mikheyev/Notebook/P. rapida partial genome/2009/11/19

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QC ligation of the clones

reagent volume conc molarity
2x 1.666666667
vector 0.333333333 50 ng/ul 26 fmol/ul
pcr 1 6 ng/ul 61 fmol/ul
t4 0.333333333 3U/ul
total 3.333333333
  • ligation overnight at 4C