User:Alexander S Mikheyev/Notebook/P. rapida partial genome/2009/11/12

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Blunt end repair of P.rapida DNA

Reagents 1x conc.
DNA 90 60ng/ul
10x 12.6 10x
ATP 12.6 10 mM
[NEB blunting enzyme] 2.7
dNTPs 12.6 10 mM
Hold 16 hours at 16C deactivate 10 min @65C program exp003 on PCR