User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/02/19

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Objective

  • Image spot plates from yesterday.
  • Make lysozyme linked AuNP's with salt added before synthesis.
  • Prepare diluted solutions of citric acid, MES, 2,2 bipyridine, NaCl, KCl, MgCl2, CaCl2 from 1 M to 100 mM, 10 mM, and 1 mM.
  • Test various concentrations to determine the minimum concentration of citric acid, MES, 2,2 bipyridine, NaCl, KCl, MgCl2, CaCl2, HEPES, and Tris that causes fiber formation.

Procedure

Creating Stock Solutions of Gold and Lysozyme

  • As seen below, each stock solution was created following the calculations in the table.
  • Each solution was created using a volumetric flask and deionized water.

Stock.2.19.png


Synthesizing Lysozyme-AuNPs

  • As seen below, each solutions was created using the following calculations.
  • We made one tube of each ratio for the following 1 M salt solutions: KCl, NaCl, CaCl2, MgCl2

2.19.png


Adding Diluted Variables to the Lysozyme-AuNP Samples

  • Dilute the stock solutions of citric acid, MES, 2,2 bipyridine, NaCl, KCl, MgCl2, and CaCl2 to 100 mM, 10 mM, and 1 mM.
  • Add 0.5 mL of each variable to the 30, 55, and 110 ratios of lysozyme-AuNPs.
  • Let sit on the bench overnight.

Images

  • Salts all form fibers or aggregates that disperse in solution but settle overtime.

Feb 19, 2014 Pic 1.jpg<br.>

Feb 19, 2014 Pic 2.jpg

  • All observations listed on yesterdays page.

pH for Samples

Screen shot 2014-02-24 at 5.33.16 PM.png