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Digestion of pSB1a3 and Tamura insert
pSB1a3 and Tamura digested with EcoRI and PstI.
Notice: This is confusing as all hell: Tamura Miniprep 1 is what we are digesting to obtain the linearized and digested pSB1a3. Turns out, we don't have any actual linear pSB1a3, so we have to obtain it from a completed Tamura miniprep that we performed ages ago.
Running the digested Tamura and pSB1a3 on a gel
Gel was 1% w/v, 40 mL. Was run for 30 minutes at 100 V.