UCLAiGEM:Notebook/MaterialsProcessing/2015/04/16

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<html><head><title>16 April 2015</title><meta content="text/html; charset=UTF-8" http-equiv="content-type"><style type="text/css">.lst-kix_kjqd8ekjqmy2-7>li:before{content:"\0025cb "}.lst-kix_kjqd8ekjqmy2-2>li:before{content:"\0025a0 "}.lst-kix_kjqd8ekjqmy2-3>li:before{content:"\0025cf "}ul.lst-kix_kjqd8ekjqmy2-0{list-style-type:none}ul.lst-kix_kjqd8ekjqmy2-1{list-style-type:none}ul.lst-kix_kjqd8ekjqmy2-2{list-style-type:none}.lst-kix_kjqd8ekjqmy2-0>li:before{content:"\0025cf "}ul.lst-kix_kjqd8ekjqmy2-3{list-style-type:none}ul.lst-kix_kjqd8ekjqmy2-4{list-style-type:none}ul.lst-kix_kjqd8ekjqmy2-5{list-style-type:none}ul.lst-kix_kjqd8ekjqmy2-6{list-style-type:none}ul.lst-kix_kjqd8ekjqmy2-7{list-style-type:none}.lst-kix_kjqd8ekjqmy2-4>li:before{content:"\0025cb "}ul.lst-kix_kjqd8ekjqmy2-8{list-style-type:none}.lst-kix_kjqd8ekjqmy2-8>li:before{content:"\0025a0 "}.lst-kix_kjqd8ekjqmy2-1>li:before{content:"\0025cb "}.lst-kix_kjqd8ekjqmy2-6>li:before{content:"\0025cf "}.lst-kix_kjqd8ekjqmy2-5>li:before{content:"\0025a0 "}ol{margin:0;padding:0}.c1{padding-left:0pt;widows:2;orphans:2;direction:ltr;margin-left:36pt}.c0{padding-left:0pt;widows:2;orphans:2;direction:ltr;margin-left:72pt}.c3{widows:2;orphans:2;height:11pt;direction:ltr}.c4{max-width:468pt;background-color:#ffffff;padding:72pt 72pt 72pt 72pt}.c2{margin:0;padding:0}.title{widows:2;padding-top:0pt;line-height:1.15;orphans:2;text-align:left;color:#000000;font-size:21pt;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}.subtitle{widows:2;padding-top:0pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-style:italic;font-size:13pt;font-family:"Trebuchet MS";padding-bottom:10pt;page-break-after:avoid}li{color:#000000;font-size:11pt;font-family:"Arial"}p{color:#000000;font-size:11pt;margin:0;font-family:"Arial"}h1{widows:2;padding-top:10pt;line-height:1.15;orphans:2;text-align:left;color:#000000;font-size:16pt;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}h2{widows:2;padding-top:10pt;line-height:1.15;orphans:2;text-align:left;color:#000000;font-size:13pt;font-family:"Trebuchet MS";font-weight:bold;padding-bottom:0pt;page-break-after:avoid}h3{widows:2;padding-top:8pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-size:12pt;font-family:"Trebuchet MS";font-weight:bold;padding-bottom:0pt;page-break-after:avoid}h4{widows:2;padding-top:8pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-size:11pt;text-decoration:underline;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}h5{widows:2;padding-top:8pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-size:11pt;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}h6{widows:2;padding-top:8pt;line-height:1.15;orphans:2;text-align:left;color:#666666;font-style:italic;font-size:11pt;font-family:"Trebuchet MS";padding-bottom:0pt;page-break-after:avoid}</style></head><body class="c4"><ul class="c2 lst-kix_kjqd8ekjqmy2-0 start"><li class="c1"><span>Centrifuged the dialyzed silk, removed flocculents</span></li><li class="c1"><span>pipetted out 500 uL onto a small weigh boat</span></li></ul><ul class="c2 lst-kix_kjqd8ekjqmy2-1 start"><li class="c0"><span>empty weigh boat: 0.569 grams</span></li><li class="c0"><span>full weigh boat + aqueous silk: 1.081 grams</span></li><li class="c0"><span>weigh boat + dry silk: 0.611 grams</span></li></ul><ul class="c2 lst-kix_kjqd8ekjqmy2-0"><li class="c1"><span>our silk solution was 0.064 g silk / mL (aka 6.4% w/v)</span></li><li class="c1"><span>NOTE: We dried the silk at room temperature, not 60 celcius, as the protocol indicated.</span></li></ul><p class="c3"><span></span></p></body></html>